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Christine Massey FOIs's avatar

Writing a book - exciting to hear!! Enjoy your well-deserved time off, Mike!!

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Mike Stone's avatar

Thank you Christine! I'm excited for both. 🙂

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Marius's avatar

Enjoy your well-deserved vacation!

I look forward to seeing more of your work after you're back.

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Mike Stone's avatar

Thank you very much Marius! I really appreciate it. 🙂

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Brian Root's avatar

I guess we are always on a "virus"-free vacation.

Except for my dang Windows PC.

Damn you Bill Gates.

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Mike Stone's avatar

Gates has those filthy claws of his in everything. 😉

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Joe Roberts's avatar

Stay away from the Bud Light. AKA Fruity Light.

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Mike Stone's avatar

🤣 Will do Joe.

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Heather Rae, INHC's avatar

Enjoy the well-earned time off. I am watching TEOC, and it is a masterpiece! Thank you for all you have done and are doing. You are (all) a lifeboat in the storm ;)

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Mike Stone's avatar

Thank you so much Heather for the kind words! I am so happy to hear that you are enjoying TEOC! I will let the rest know! We love the feedback. 🙂

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Jeffrey Strahl's avatar

Yeah for the book. Have fun doing recharging, Mike!!

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Mike Stone's avatar

Thank you Jeffrey! I will do my best. 🙂

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Greg C's avatar

Enjoy! But I just had to post this conversation with an AI ChatBot I encountered today:

"So has a "Virus" ever been seen invading a cell in vitro versus outside the body?"

Yes, viruses have been observed invading cells in vitro, which means in a controlled environment outside of a living organism, such as in a petri dish or test tube. This is a common method used in virology, the study of viruses.

Viruses are incredibly small, much smaller than the cells they infect, and can’t be seen with a regular light microscope. However, with the advent of electron microscopy, which uses a beam of electrons instead of light to create an image, scientists have been able to visualize viruses and their interactions with cells in great detail.

In a typical scenario, a virus attaches to a specific receptor on the surface of a cell, and then injects its genetic material into the cell. This genetic material uses the cell’s own machinery to replicate, producing more viruses. Eventually, the cell bursts, releasing the new viruses to infect more cells. This process has been observed and documented in numerous studies.

It’s important to note that while in vitro studies provide valuable insights, they don’t fully replicate the complex interactions that occur within a living organism. Therefore, in vitro findings are often complemented by in vivo studies, which are conducted in living organisms.

Would you like to learn more about how viruses work, or is there another topic you’re interested in exploring?

"Let me rephrase. Has a virus been seen infecting a live cell that is part of a live organism. not in some external dish?"

Yes, viruses have been observed infecting cells within live organisms, a process known as in vivo infection. However, due to the microscopic size of viruses and the complexity of living organisms, these observations are technically challenging and often require the use of advanced imaging techniques.

One such technique is intravital microscopy, which allows for the visualization of biological processes in live animals. Using this method, scientists have been able to observe viral infections in real-time within living organisms. For example, studies have visualized how the influenza virus infects the respiratory tract in mice, or how the Zika virus infects neural stem cells in the brains of live mice.

Another technique is the use of genetically engineered viruses that produce fluorescent proteins. These “glowing” viruses can be tracked under a microscope as they infect cells within a living organism.

It’s important to note that while these observations provide valuable insights into how viruses infect cells and cause disease, they represent a simplified view of a very complex process. The actual process of viral infection involves many steps and is influenced by numerous factors, including the type of virus, the type of cell, the presence of immune cells, and the overall health of the organism.

Would you like to delve deeper into this topic, or is there another aspect of virology or biology you’re interested in?

"Please define "visualization" as used in your above statement."

In the context of the previous statements, “visualization” refers to the process of making something perceptible to the eye, especially something that is typically too small or complex to see with the naked eye. In scientific research, visualization often involves the use of specialized equipment or techniques to observe and record phenomena that are otherwise invisible or difficult to understand.

For example, in the study of viruses, visualization can involve the use of electron microscopes, which use a beam of electrons instead of light to magnify an object up to a million times its actual size. This allows scientists to see the structure of viruses in great detail.

In the case of observing viruses infecting cells within a living organism, visualization might involve techniques like intravital microscopy, which allows for real-time, high-resolution imaging of biological processes within a living organism. Or it might involve the use of genetically engineered viruses that produce fluorescent proteins, making the viruses “glow” under certain types of light and thus allowing them to be tracked as they move through an organism and infect cells.

In all these cases, the goal of visualization is to make the invisible visible, providing insights into biological processes that help us understand how life works at the microscopic level.

Would you like to explore more about these visualization techniques or any other topic?

"Can you show me a link to a video of intravital microscopy actually happening?"

While I, as an AI, am unable to provide direct links, I can guide you on how to find such resources. You can find videos of intravital microscopy in action by searching for terms like “intravital microscopy video” or “intravital microscopy in vivo imaging” on video-sharing platforms like YouTube or on scientific websites.

These videos often show cells and tissues in real-time, and some may show the process of viral infection. However, please note that interpreting these videos often requires a certain level of scientific knowledge, as they can be quite complex and technical.

If you’re interested in a specific type of intravital microscopy or a specific application (such as observing viral infections), including those terms in your search may help you find the most relevant videos.

Would you like to know more about intravital microscopy or any other topic?

"My search of "intravital microscopy" videos do not show any videos that show a viral particle. So, you may have been misled."

I apologize if there was any misunderstanding. Intravital microscopy is indeed a powerful tool for visualizing biological processes in real-time within living organisms.

However, due to the extremely small size of viruses (much smaller than most cells), visualizing individual viral particles infecting cells in a live organism is beyond the resolution of most current imaging techniques, including intravital microscopy. <------ BINGO!!!!!!!!!!!!!!!!!!!!!!!!!

Typically, studies using intravital microscopy visualize the effects of viral infection, such as cellular responses, rather than the viruses themselves. For direct visualization of viruses, techniques like electron microscopy are used, but these are generally not applicable to live organisms due to the preparation and conditions required for these types of microscopy.

The field of virology often involves combining multiple techniques to study viruses, their effects on cells, and the immune response. This can include genetic techniques, in vitro cell culture studies, animal models of infection, and various types of microscopy.

I hope this clarifies the matter. If you have more questions or if there’s another topic you’d like to explore, feel free to ask.

"Bingo! Thanks!"

You’re welcome! I’m glad I could assist you. If you have any more questions or if there’s another topic you’d like to explore, don’t hesitate to ask. Happy exploring!

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